Identification and Characterization of MicroRNAs in Asiatic Cotton (Gossypium arboreum L.)

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Identification and Characterization of MicroRNAs in Asiatic Cotton (Gossypium arboreum L.)

To date, no miRNAs have been identified in the important diploid cotton species although there are several reports on miRNAs in upland cotton. In this study, we identified 73 miRNAs, belonging to 49 families, from Asiatic cotton using a well-developed comparative genome-based homologue search. Several of the predicted miRNAs were validated using quantitative real time PCR (qRT-PCR). The length ...

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Identification and Characterization of miRNA Transcriptome in Asiatic Cotton (Gossypium arboreum) Using High Throughput Sequencing

MicroRNAs (miRNAs) are small 20-24nt molecules that have been well studied over the past decade due to their important regulatory roles in different cellular processes. The mature sequences are more conserved across vast phylogenetic scales than their precursors and some are conserved within entire kingdoms, hence, their loci and function can be predicted by homology searches. Different studies...

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Introgression of cotton leaf curl virus-resistant genes from Asiatic cotton (Gossypium arboreum) into upland cotton (G. hirsutum).

Cotton is under the constant threat of leaf curl virus, which is a major constraint for successful production of cotton in the Pakistan. A total of 3338 cotton genotypes belonging to different research stations were screened, but none were found to be resistant against the Burewala strain of cotton leaf curl virus (CLCuV). We explored the possibility of transferring virus-resistant genes f...

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Identification and characterization of CONSTANS-like (COL) gene family in upland cotton (Gossypium hirsutum L.)

The CONSTANS/FLOWERING LOCUS T (CO/FT) regulon plays a central role in the control of flowering time in photoperiod-sensitive plants. Flowering time in wild cotton (Gossypium spp.) has strict photoperiod sensitivity, but domesticated cotton is day-neutral. Information on the molecular characterization of the CO and CO-like (COL) genes in cotton is very limited. In this study, we identified 42 C...

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Construction of Genetic Linkage Map and QTL Analysis for Fiber Traits in Diploid Cotton (Gossypium arboreum x Gossypium herbaceum)

Diploid A genome cottons are grown for their natural fiber in drought prone areas of Asia. Although they possess inferior fiber qualities to cultivated Upland and Pima cotton, their value lies in their inherent resistance to pests and diseases. Molecular linkage maps provide essential tools for plant genetic research, facilitating quantitative trait locus (QTL) mapping, marker-assisted selectio...

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ژورنال

عنوان ژورنال: PLoS ONE

سال: 2012

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0033696